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Description

Senescent cells acquire an irreversible senescence-associated secretory phenotype (SASP)

Iwahashi et al., 2002

RT-qPCR was carried out using the 2 Hieff UNICON Universal Blue qPCR Master Mix (Yeasen, Shanghai, China) under the following amplification conditions: Uracil-DNA Glycosylase (UDG) activation at 50 C for 2 min, initial denaturation at 95 C for 2 min, then 40 cycles of 95 C for 15 s and 60 C for 30 s for denaturation and annealing/extension, respectively, concluding with a melting curve analysis