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Hypothermic preservation Hypothermic preservation at temperatures between 4C and 10C allows for the elimination of debris, toxic metabolites, and free radicals produced during hypothermia that would otherwise accumulate during cold static storage ( However, due to the hypothermic state, the metabolic activity of the organ is dramatically impeded during perfusion restricting functional organ assessments ( + /K + pumps ( ex vivo therapies as a promising approach during EVMP is thus restricted due to diminished exposure times of the graft during HMP and compromised pharmacodynamics at low temperatures