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Membranes were blocked with 5% skimmed milk in Tris-buffered saline containing 0.1% Tween 20 for 1 hour at room temperature and incubated with primary antibodies overnight at 4 C

The healthy NP is primarily comprised of highly gelatinous and hydrophilic material that is a combination of negatively charged proteoglycans, collagen, and non-collagenous proteins

doi: 10.1111/cen.14189 54 BauerMWhybrowPC

322 This process is essential for the maintenance of mitochondrial integrity, as impairment of mitophagy by PINK1 or Parkin disruption contributes to increased oxidative stress, exacerbating hepatic steatosis and inflammation