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Following incubation, the resin was washed thrice on a midi batch purification column (Generon, Maidenhead, UK) using wash buffer (20 mM Tris-HCl, pH 7.8, 0.5 M NaCl, 5 M urea, 10-50 mM imidazole) before elution in buffer containing 20 mM Tris-HCl, pH 7.8, 0.5 M NaCl, 300 mM imidazole and 5 M urea