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Such substituents can include, for example, a halogen, a deuterium, a hydroxyl, a carbonyl (such as a carboxyl, an alkoxycarbonyl, a formyl, or an acyl), a thiocarbonyl (such as a thioester, a thioacetate, or a thioformate), an alkoxyl, a phosphoryl, a phosphate, a phosphonate, a phosphinate, an amino, an amido, an amidine, an imine, a cyano, a nitro, an azido, a sulfhydryl, an alkylthio, a sulfate, a sulfonate, a sulfamoyl, a sulfonamido, a sulfonyl, a heterocyclyl, an aralkyl, an aromatic, or heteroaromatic moiety

Cytoskeletal proteoforms enriched in frail brain, including DBN1, TUBA8, PALM and NEFM might represent degradation of full-length scaffolding proteins, indicating neuronal damage

doi: 10.1038/s41598-018-30875-y 57 LeeN

The cells were then harvested and aliquoted into 100 l portions to 96-well plates, each well containing 3 10 5 cells