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2 Materials and methods 2.1 Experimental animals This study comprised adult male C57BL/6 mice (aged 810 weeks) weighing 2228 g (Shanghai Jihui Experimental Animal Breeding Co., Ltd.), which were housed for at least 1 week in advance in an animal room with alternating cycles of 12 h of light and 12 h of darkness (lights on at 7:00 a.m