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We used the cryo-EM structure of the FBXO22-UNC10088 C -NSD2 complex to prepare 3 different systems in addition to one already published structure of FBXO22-BACH1 (PDB: 8UA6) 13 , leading to a total of four FBXO22 configurations, such as FBXO22 alone, FBXO22-UNC10088 C , FBXO22-UNC10088 C -NSD2, and FBXO22-BACH1

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The actual amino acid sequence, the concentration per vial, and the sterility of the solution can vary between research suppliers and between batches from the same supplier

The fluorescence of the probe increases with the decrease of oxygen in a closed environment, and the oxygen consumption rate of cells is judged by detecting the change of the fluorescence value