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Following this, the sections were incubated at room temperature with 10% goat serum for 30 minutes

The reaction was initiated by mixing 2035 g protein with a reaction mixture containing 10 mM ATP, 5 mM L-cysteine, 50 mM L-glutamate, 500 M acivicin, 20 mM MgCl 2 , 100 mM Tris-HCl pH 8.2 in a total volume of 150 l followed by incubation for 15 min at 25C

This suggests that NP4 could effectively promote the polarization of M2-type macrophages towards M1-type macrophages, thereby alleviating immune suppression

[DOI] [PMC free article] [PubMed] [Google Scholar] 7.Wang L., Zhou J., Hou Y., Yi D., Ding B., Xie J., Zhang Y., Chen H., Wu T., Zhao D., et al