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Three clones were picked and transferred into 5 mL LB liquid medium supplemented with 100 M ampicillin sodium (Amp) and 50 M spectinomycin dihydrochloride pentahydrate (Spe), and incubated at 37 C, 220 rpm for 6 h in an orbital shaking incubator

Good providers adjust formulations based on your health conditions, goals, and previous responses

10.1038/nrm3099 Nat

Folic acid (FA)-induced AKI is one of the typical models simulating drug or toxicant-induced tubular injury, which is closely related to crystal formation in the tubule lumen and cellular oxidative stress, leading to massive inflammatory reaction and eventually tubular cell death (Martin-Sanchez et al., 2018)