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Analytical size-exclusion chromatograms and corresponding SDSPAGE analyses are shown in Extended Data Fig

4.1 Acute liver failure ALF is an intractable clinical syndrome with multiple causes featured by a fast reduction in hepatic function, increased aminotransferases, altered mentation, and disturbed coagulation in the absence of chronic liver diseases

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Although vitamin B12 is water-soluble and excess amounts are typically excreted in the urine, the body still stores significant quantities (primarily in the liver) with its levels continuously regulated