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(11) You must note the fact that you used a breath tube device in Step 3 of the ATF

TMV can multiply only inside a living cell, but it can survive in a dormant state in dead tissue, retaining its ability to infect growing plants for years after the infected plant part died

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Three standard reproductive assays of PG have been performed, all in male and female mice, using repeated doses and multigeneration assessment for reproductive outcomes (Kavlock et al., 1987