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Our objective was to determine whether -blocker treatments that easily cross the bloodbrain barrier reduce the risk of Alzheimer's disease compared to less permeable -blockers

DIV12 the neurons were fixed (4% paraformaldehyde, 3% sucrose, 60 mM PIPES, 25 mM HEPES, 5 mM EGTA, 1 mM MgCl 2 , pH 7.4) for 20 min at room temperature and mounted