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The most common of these are increased body fat (particularly abdominal fat), decreased lean body mass (including muscle), functional strength, thin skin and cool extremities, decreased psychological well-being and energy, reduced bone density, an increase in c-reactive protein, low-density lipoprotein (LDL), fibrinogen, plasminogen activator inhibitor-1 (PAI-1), a decrease in high-density lipoprotein (HDL), decreased insulin sensitivity, and decreased quality of life [25]

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After layering, the lower layer extract was collected and dried to obtain the total lipids

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