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GLAST-Cre ERT2 mediated DNA recombination can be used to quantify astroglial cell numbers If Cre ERT2 is expressed in a cell type-specific manner, the proportion of this cell type in a given region can be determined by qRT-PCR across a single loxP site of the non-recombined locus, since reduction of amplimer levels indicates successful gene deletion (Fig

While not every peptide is equally sensitive, gentle swirling is a simple precaution that minimizes unnecessary stress without slowing the reconstitution process

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