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Tutwiler V, Mukhitov AR, Peshkova AD, Le Minh G, Khismatullin RR, Vicksman J, et al

Algal production of nano-silver and gold: Their antimicrobial and cytotoxic activities: A review

Beads were washed with 20 mM and 40 mM imidazole steps and protein was eluted using 250 mM imidazole in 50 mM Tris-HCl, 100 mM NaCl, 0.5 mM MgCl 2 (pH 7.5)