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It is possible to repeat these four steps to remove the preceding PLS predictors and add additional PLS predictors, but we choose to consider only one potential PLS predictor for the sake of keeping our model as simple and interpretable as possible and because this methodology is prone to overfitting when multiple PLS components are retained

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54 ) were cultured in DMEM medium with 10% fetal bovine serum and transfected with a pCMV(d1)-PINK1-3HA plasmid for 14 h at 37 C and 5% CO 2 in a humidified atmosphere