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Mutations in AR receptor, AR overexpression, intracrine production, contorted expression of enzymes and cofactors of AR, autonomous activation of AR signaling by cytokines in the absence of androgen ligands, and the presence of multiple splice variants of AR are the main causative agents responsible for the therapeutic failure [12, 13]

Secondly, an 8-week follow-up duration may not be sufficient to observe long-term metabolic and clinical changes (e.g., the development of other medical co-morbidities)

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FBS, DMEM, and tissue culture reagents were obtained from Invitrogen (Carlsbad, CA, USA)