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In general, detection methods are based on the use of fluorescence microscopy (Zhang et al., 2022), flow cytometry (Kauffman et al., 2016) or spectrofluorometer (Steenbergen et al., 1997

25 Ascorbate is an excellent antioxidant in cells, not only because of its relatively high concentration but also because of the high rate constant of its reaction with free radicals

These findings all indicate that ferroptosis promotes the development of DCM through abnormal iron metabolism, lipid peroxidation, ROS production, and enhanced cardiomyocyte inflammation (Figure 1)

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