Cultures were then diluted 1:500 to 1:1,000 in TSB or Seahorse XF DMEM (Agilent) supplemented with 100 mM glucose, 2 mM L-glutamine and 1 mM sodium pyruvate, and dispensed into poly-D-lysine (PDL)-coated Seahorse XF HS miniplates (100 l per well)
One-time + setup costs (typical ranges to get launched): Legal and entity formation (LLC/PC/MSO structure, contracts, etc.): $2,000+ Provider licensing and credentialing (varies sharply by number of states and whether you use a partner): $2,000+ LegitScript certification and merchant setup: roughly $2,000 to $3,000 to start Business and clinical insurance (malpractice, cyber, general liability): $2,000+ per year Brand, domain, and website: a couple hundred dollars to $2,000+ depending on whether you DIY or hire out (and website platform pricing) Initial software configuration and integrations: often bundled into monthly costs That puts a lean, single-specialty virtual weight loss clinic in roughly the $10,000 range to launch, with multi-state and more clinically complex builds potentially running higher
W., Lee, J., Park, S
Single-use supplies, clean prep, and a brief check-in keep things streamlined
