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The samples were desalted using a Zeba spin desalting column (Thermo Fisher Scientific) and 100 l of the desalted sample was combined with 100 l of 100 M NADPH

In addition, the beneficial bacterium up-regulated the expression of several defense genes encoding PR-10a, the antioxidative monodehydroascorbate reductase enzyme and two GSTs (Maketon et al., 2012)