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However, high doses of food additives may cause pharmacologically an adverse effect in a small percentage of children with ASD [92]

[5] Disruption of these pathways leads to accumulation of homocysteine and methylmalonic acid, metabolic signatures linked to megaloblastic anemia and demyelinating neuropathy

The oligonucleotides were supplied by Metabion GmbH (Planegg/Steinkirchen, Germany), and the PCR kit containing ALLin HiFi DNA Polymerase was purchased from highQu GmbH (Kraichtal, Germany) and used according to the manufacturers instructions

Nacalai Tesuque Corporation, Kyoto, Japan), the membrane was washed three times with Tris-buffered saline (TBS-T), soaked in primary antibody, and incubated overnight at 4 C