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We performed densitometry analysis to calculate the intensity of phosphorylated and total protein using ImageJ software

Moreover, although preclinical studies have yielded promising results, large-scale, double-blinded clinical trials remain necessary to verify the therapeutic efficacy and feasibility of these approaches in DN patients

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In melanoma and breast cancer mouse models, it has been observed that mice fed a low-protein diet (4% kcal protein) exhibit reduced IGF-1 levels and slower tumor progression compared to those fed a high-protein diet (18% kcal protein)