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2024;22(1):102e112109

10.1007/s00415-020-09723-5 [DOI] [PubMed] [Google Scholar] Lubomski M., Xu X., Holmes A

A denaturing agent, such as 8M urea, will also break the interaction by altering the configuration of the site where the antigen binds molecule of the antibody 4 Step : Affinity medium is re-equilibrated with binding buffer