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Trypsin was neutralized with 500 l complete medium, the cells were resuspended and 100 l of the suspension was mixed with 6.9 ml Beckman Coulter Isoton II diluent (Fisher Scientific, NC2470899) for counting with a Beckman Coulter Z1 Coulter Cell Particle counter

Priority should also be given to studies that apply standardised outcome measures, including UPDRS with MCID reporting, DAT imaging, and validated oxidative stress biomarkers

LDH was measured in both the culture medium and cell lysates, and LDH release (%) is expressed as (LDH levels in culture medium)/([LDH levels in culture medium] + [LDH levels in cell lysis]) 100