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Plates were washed five times with HBS + 0.1% Tween-20, and 100 l of streptavidinhorseradish peroxidase (HRP) was added to each well, followed by another one-hour incubation at room temperature

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When exercise interventions exceed 16 weeks, there is no significant impact on the activity of SOD, CAT, and GPX

Mitochondrial reactive oxygen species release is unaltered by IF In addition to determining changes in functional parameters, we measured the release of mitochondrial H 2 O 2 , a relatively stable and membrane-permeable ROS often used as a marker for oxidant production (Fig