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Either Ub2, Ub4, or Ub8 (1.0 g) was incubated with OTUD5 (0.5 g or the indicated amount) (37 C, 4 h or for the indicated duration) in the reaction buffer [100 mM Hepes (pH 7.5), 5 mM EGTA, 0.5% NP40, 10 mM EDTA, and 50 mM DTT]

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