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Intracellular GSH levels A2780 and A2780DDP cells were incubated for up to 24 h with 50 M of buthionine sulphoximine (BSO), which inhibits the GSH synthesis, and the intracellular GSH was measured (Figure 3)

In HCECs applied with desiccating stress, the CEC ferroptosis was triggered by the aggregation of autophagy impairment-derived sequestosome 1 (SQSTM1), the overexpression of which fostered ferroptotic changes and substantially elevated ACSL4 (124)