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Rapid glucocorticoid-mediated endocannabinoid release and opposing regulation of glutamate and -aminobutyric acid inputs to hypothalamic magnocellular neurons

For method 2 sample preparation, the initial sample preparation was the same as described for method 1, except that NEM was added to the extraction solvent (50 L of 50 mM NEM in 80% methanol, 20% 10 mM ammonium formate, pH 7.0 and 50 L of 100% ACN)

7.3.4 Clinical implications and problems The potential of probiotics as a therapeutic strategy for mitigating BBB dysfunction and cognitive decline is supported by promising preclinical findings, but clinical evidence remains limited (Sproten et al., 2024)