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All data were analyzed by one-way or two-way analysis of variance followed by Student's modified t test with Bonferroni corrections for multiple comparisons between means using the modified error mean square term (EMS) from the ANOVA 103

It is possible that this late change in amount and properties of microvesicles from stored RBC may be the major reason for why long stored blood units were primarily responsible for the deleterious effects in transfused patients

They were kept in a controlled environment (at 22C 2C with 5060% relative humidity) during a 12-h light-dark cycle

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