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Moosmayer D, Hilpmann A, Hoffmann J, Schnirch L, Zimmermann K, Badock V, et al

4ad, genomic DNA was extracted according to Qiamp Fast DNA Stool extraction kit (Qiagen) and the V3V4 region of the bacterial 16S rRNA gene was amplified with the NEBNext Q5 Hot Start Hifi PCR Master Mix (NEB) using a dual-index strategy