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Another 1 ml aliquot of the supernatant was neutralized with 1.5 ml of 0.5 M phosphate buffers, 50 l of 2-vinylpyridine was added to mask GSH, and the constants of the tube were mixed until an emulsion formed

While proteomics measures hundreds of proteins in clinical samples, the complex data processing and interpretation remain a significant challenge 8,9

GST Tag Interference with Protein Function Challenge : In some cases, the GST tag can interfere with the function of the target protein, particularly if the protein's active site or interaction domains are close to the fusion point

Abstract Glucose 6-phosphate dehydrogenase deficiency (G6PD-d) is the most common enzymopathy in the world, occurring in 58% of the global population (half a billion people)