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Description

The beads remain suspended in the sample solution during mixing, allowing the GST-tagged protein to bind to the immobilized ligand

Q: What precautions should be taken before and during the treatment process

ESA (PDB ID: 6U4X) 26 and BSA (PDB ID: 4F5S) 27,28 were prepared using Schrodinger protein preparation wizard and all ionizable residues were set to their probable protonation state at pH 7.4 using ProPka 29