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RSL3 inhibits mTOR signaling in thyroid cancer cells The mTOR signaling pathway has recently been implicated in counteracting ferroptosis by regulating ROS production 20 , therefore, it was important to examine the effects of RSL3 on mTOR signaling pathway and its downstream proteins and whether inactivation of mTOR pathway signaling will occur in thyroid cancer cells with GPX4 inhibition

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Under conditions of oxidant attack, GSH is rapidly converted to GSSG, making the elevation of the intracellular GSSG/GSH ratio a widely accepted marker for oxidative stress