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Although RB loss confers a certain level of resistance to CDK4 inhibitors, knockdown of RB alone couldnt fully overcome cell cycle arrest induced by CDK4 inhibitors, as shown before 11

To allow for synchronised development with constant density, embryos were collected on agar plates (2.3% agar, 1% sucrose, and 0.35% acetic acid) with live yeast paste and directly added to the standard diet in a bottle with a fixed volume

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