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Then, the media was removed and cells were washed twice with PBS

Afterwards, the solution containing the refolded protein was purified via SEC in refolding buffer 2 on the kta Avant or Go system (Cytiva) with a Superdex 75 Increase 10/300 GL or HiLoad 16/600 Superdex 75 pg column (Cytiva) to check for and remove aggregates at a flow rate of 0.5 or 1 mL/min

Ingredients like GHK-Cu align perfectly with this philosophy

Markus Vogel, Head of Bioconjugation Research (Germany) "We needed a cyclic disulfide linker tailored to a specific antibody that would enhance conjugation efficiency without compromising immunoreactivity