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ZnT2 is a critical mediator of lysosomal-mediated cell death during early mammary gland involution

Ther Apher Dial

QuirogaJAlarconPRamirezMFManosalvaCTeuberSCarrettaMDet al

In short, genomic DNA was extracted by cell lysis (10 mM Tris-HCl, 10 mM EDTA, 150 mM NaCl and 0.1% SDS), proteinase K treatment (New England Biolabs) and DNAse-free RNAse digest (Thermo Fisher), followed by two rounds of phenol extraction and isopropanol precipitation