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Notably, RT-qPCR analysis showed a sharp increase in OCT4 mRNA expression, a marker of pluripotent stem cells and essential for TIC self-renewal [33], in the colonies formed in 3D gels relative to 2D culture conditions (Fig

Biological analyses were performed on serum, measuring folate, vitamin B12, homocysteine, and folate receptor-alpha autoantibodies using radioimmunoassay and mass spectrometry