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Identification of hmox1 as a critical ferroptosis-related gene in atherosclerosis

Obradovic T, Dobson GG, Shingaki T, Kungu T, Hidalgo IJ

Ice-cold phosphate-buffered saline (PBS, pH 7.4) was incorporated at a 1:10 (w/v) ratio, and samples were homogenized thoroughly with a mechanical homogenizer on ice

The study found a 75% reduction in erythrocyte glutathione reductase activity in pigs on the riboflavin-free diet, with no change in reduced glutathione levels