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Discovery of a long-chain carbamoyl aminocarnitine derivative, a reversible carnitine palmitoyltransferase inhibitor with antiketotic and antidiabetic activity

Experiments have verified that this strategy significantly enhances tumor killing efficacy and inhibits recurrence by simultaneously overcoming radiotherapy/chemotherapy resistance (reducing H2O2 and GSH levels), inducing mitochondrial dysfunction (ROS accumulation), and activating cuproptosis (DLAT oligomerization and lipid peroxidation) (Zhu et al., 2025)

There is an ongoing phase 2/3 randomised, double-blind, placebo-controlled trial (NCT06126315) of the biological and clinical effects of acetyl-l-carnitine planning to recruit ~250 patients [88]

Cells were then washed and stained for intracellular markers 30 min at 4C and acquired by CytoFLEX LX Flow Cytometer (Beckman Coulter, RRID: SCR_025067)