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The apolipoprotein E4 genotype contributes to the abnormal accumulation of lipid droplets (LD) in microglia and the formation of neurotoxic LD-accumulating microglia, which are capable of directly inducing neuronal Tau phosphorylation and apoptosis, and the degree of LD accumulation is positively correlated with the severity of cognitive decline and pathology [148]

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2.7 Enzyme-linked immunosorbent assay (ELISA) After euthanasia, mouse heart blood was collected, incubated at room temperature (23C 2C) for 2 h, and centrifuged at 13,500 g for 10 min at 4C

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