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To obtain PitNET tissue-derived free-floating spheres, cells were grown in DMEM-F12 (Thermo Fisher Scientific, USA), containing 1 penicillin/streptomycin solution, 20 ng/ml epidermal growth factor (EGF) (Sigma-Aldrich, Germany), 10 ng/ml basic fibroblast growth factor (bFGF) (Sigma-Aldrich, Germany), and 1 B27 supplement (GIBCO, USA)

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Positive selection The survival of developing lymphocytes with 'desirable' antigen receptors is favoured by the processes of positive selection, which include cessation of V(D)J recombination and developmental progression

Theyre also available in supplement form