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Bilirubin must be conjugated before it can be excreted into bile (conjugation makes bilirubin water soluble, because it breaks apart the hydrogen bond within the molecule, which keep it hydrophobic and in so doing, makes the central methylene group available to the diazo reagent for measurement)

Enhanced radiosensitivity by inhibition of nuclear factor kappa B activation in human malignant glioma cells Int J Radiat Biol 1997 72 : 157162 Zhou G, Kuo MT

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In contrast, another study found no difference in micronucleus levels between patients who received MTX treatment and those who did not, suggesting that the generation of micronuclei was associated with RA itself (47)