Following endocytosis, the HoloTC complex is degraded in lysosomes and free B12 is released into the cytoplasm, via CblF (Rutsch et al., 2009) and CblJ ( This free cytoplasmic B12 undergoes a series of intracellular transport and modifications to enable two core enzymatic reactions: 1) adenosyl-B12 acts as a co-factor for the enzyme methylmalonyl-coA mutase that catalyzes the conversion of methylmalonyl-coA to succinyl-coA, thereby enabling restoration of tricarboxylic acid (TCA) cycle intermediates and 2) methyl-B12 enables methionine synthase in the re-methylation of homocysteine to methionine using methyl-tetrahydrofolate (MTHF) as a methyl donor, thereby enabling the vital biochemical pathways of the folate cycle and methionine cycle, facilitating the downstream synthesis of important compounds like DNA and RNA (Figure 2)
That is a genetic-knockdown study in mice, not a drug trial: it tells you the target is real and metabolically meaningful in an animal model, but it does not test a drug, a dose, or a route
5-Amino-1MQ Dosage Chart | Quick Breakdown What is 5-Amino-1MQ 5-Amino-1-methylquinolinium (5-amino-1MQ) is a small molecule that is studied for its potential influences on body weight, metabolic health, and possibly cancer progression
Treatment is provider-guided and based on lab results to ensure safe, effective dosing
