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Purification of fusion proteins by affinity chromatography on glutathione resin

Both siblings had the same homozygous 22-bp deletion in the cytoplasmic domain of the GHR, resulting in a frameshift and premature stop codon The resultant GHR was truncated at amino acid 449 (GHR1-449) after Box 1, the JAK2 binding domain of the receptor, and functional studies in HEK293 and Chinese hamster ovary cells showed a selective loss of STAT5 signaling in cells expressing GHR1-449 (Milward et al., 2004)

A pragmatic randomized controlled trial of thiopurine methyltransferase genotyping prior to azathioprine treatment: the TARGET study

PMID 9705830