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Samples were thawed on ice, diluted 5-fold with diluent, and 50 L aliquots were assayed in duplicate using the solid phase Mouse Thrombopoietin Quantikine ELISA Kit (Biotechne, Minneapolis, MN catalog #MTP00), quantified to a standard control curve displaying an R 2 = 0.999

Analyzing mtDNA copy number and integrity through qPCR and long-range PCR techniques [116] in hEDS patient samples could reveal potential mitochondrial DNA alterations associated with the condition

For instance, salinity stress leads to increased activities of mitochondrial and peroxisomal MDHARs in Lycopersicon pennellii , which is a salt tolerant wild variety (Mittova et al., 2003)

Unlike the traditional one drug-one target-one disease paradigm, network-based drug discovery reveals disease mechanisms from a holistic perspective, fully considering the complexity of biological systems [10]