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It has three major domains: a) variable N-terminal domain (421 amino acids), b) central DNA binding domain (65 amino acids), and c) C-terminal domain (250 amino acids)

It is possible to feel a slight sensation during treatment, but patients typically do not require any anesthetics

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The blue color is a useful spectroscopic and visual handle for monitoring copper complexation, for assessing the integrity of the complex in solution, and for distinguishing the copper-bound form from the apo-peptide (which is colorless or pale)