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Validation of the method and stability analyses Accuracy, precision and recovery of the method were determined on 3 separate days in blood that was haemolyzed by 1:3 (v/v) dilution with H 2 O

Alcohol consumption can increase brain oxidative stress through various mechanisms, mainly due to its metabolism by CYP2E1 enzyme and the subsequent production of ROS ( Mitochondrial uncoupling protein 2 (UCP2) is an anion transporter located in the inner mitochondrial membrane ( UCP2 gene is located on chromosome 11 (11q13) ( UCP2 variant is the missense variant rs660339 in exon 4, a C to T transition, resulting in an amino acid substitution at position 55 of the UCP2 (Ala55Val) which seems to modify the uncoupling degree and consequently protein activity ( UCP2 variants may be tissue depended ( UCP2 rs660339 variant with migraine susceptibility and clinical phenotypes

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4c) and the resulting assembloids closely mirrored the cellular composition and proportions of tissue (Fig