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Next, we monitored autophagic flux in GSH-depleted cells by using a tandem fluorescent-tagged LC3 lentivirus mRFP-GFP-LC3 expression system

However, it is impossible to correlate the intake of polyphenols present in a peculiar food and correctly evaluate the subsequent amount of polyphenols absorbed: besides environmental factors, such as light, temperature, water availability, nutrient status, and biotic stress, seasoning, cultivar, food processing - cooking, frying, toasting, etc