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Auria BioBank samples were coded and accompanied with information about gender, age, diagnosis and time interval between diagnosis date and sample collection

In comparison, the sample flow-through speed through spin columns or filter plates is very fast and difficult to control

Morning Fasted Administration (68 AM) Insulin sensitivity peaks in the early morning due to overnight fasting (812 hours without caloric intake) and cortisol-driven hepatic glucose output

After removal of unbound proteins by PBS washing, immobilised IgG [anti-GliT] was eluted using 100 mM glycine pH 2.8, followed by immediate neutralization using 100 mM Trizma base pH 8.3