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10.1016/j.phytochem.2017.08.004 12 ChenM

Deresinski, SC

Recombinant proteins were expressed in Escherichia coli BL21-CodonPlus (DE3) cultured in LB medium, after induction with 0.2 mM IPTG overnight at 16 C, the bacteria were pelleted by centrifugation at 4000 g for 10 min